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To this end, FH-deficient cells were incubated with uniformly labelled 13 C 5 -glutamine, which generates 13 C 5 -labelled glutamate and 13 C 4 -labelled fumarate, and the isotopologue distribution of succinicGSH was analysed by LC-MS (see Fig

This is precisely why some researchers prefer the short-acting no-DAC form when the goal is to reproduce a physiologic pulse, and why the long-term consequences of the DAC forms sustained pattern in humans remain an open question rather than a settled benefit

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Unlike the self-polymerization approach, requiring high alkalinity and long reaction times, the enzymatic method achieves fast polymerization under mild conditions, which broadens its range of substrates and applications (Magalhes et al., 2022)