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Description
RT-PCR was done using a standard SYBR-green PCR kit (SYBR Premix EX TaqTM II, Takara), and the gene-specific PCR amplification was conducted using the Applied Biosystems StepOnePlus Real-Time PCR System (Applied Biosystems, USA)
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however, the number of steps required for reduction in NAS score/liver enzymes has not been evaluated
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Science 354 , 572577 (2016)
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Nat Rev Neurosci 19: 6380, 2018
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