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While most RecA inteins, including I-CM have an optimal cleaving range between pH 6 and 7.5, gp41-1 was found to be independent of pH between pH 6 and 9, and only showed a significant drop in activity at extreme pH values of 4 and 10

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doi: 10.3791/3482 126 BasogluHGoncuBAkbasF

where they have enriched monocytes from healthy volunteers to generate the functional genomics datasets reanalyzed herein ( Cell Culture Monocytes were cultured in complete media consisting of Dulbeccos Modified Eagle Medium containing high glucose and sodium pyruvate (ThermoFisher Scientific) with 10% heat-inactivated fetal bovine serum (Wisent BioProducts) and 100U/mL penicillin/streptomycin (Corning)