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Following three additional washes with PBS buffer, the cells were collected and analyzed by flow cytometry (FACS, Beckman Coulter, Fullerton, CA, USA)

Nanotechnology, a rapidly expanding field with applications in health and disease, is being explored to develop safe and effective therapies to reduce the symptoms of AD post topical and systemic administration
An enhanced chemiluminescence (ECL) detection system (Amersham) was used

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