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Description
Leucine is a catalytic substrate for GDH and an allosteric activator of GDH as well
Cellular drug treatments To investigate protein degradation, proteasome was inhibited with 5 M MG132 (Sigma, M7449) or lysosomal autophagy inhibited with 10 M chloroquine (Sigma, C6628) for 24 h
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The paper proposed that GHK works partly by helping copper get into cells - the idea behind studying the copper-bound form, GHK-Cu, rather than the peptide alone
