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Amino acid profiling siblings discordant for konzo identifies deficiencies in key sulfur amino acids and suggests mitochondrial distress To determine if siblings discordant for konzo had major differences in amino acid profiles which may contribute to changes in GPx3 abundance, we used an HPLC-based approach to quantify plasma amino acids in samples from study participants

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Cell line identity was confirmed at the time of experimentation by short tandem repeat genotyping and compared with our database of cell line short tandem repeat profiles (www.TXCCR.org)

Reviewing solvent compatibility, pH, handling procedures, and storage conditions often resolves the issue