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To overcome this limitation, NCs have been investigated as a promising alternative due to their regenerative properties [22]

The threshold cycle value (CT) was determined for each measurement, and mRNA expression was calculated relative to the control using the comparative critical threshold (2 CT) method, where CT = CTmRNA CThousekeeping control
In the cerebellum, EAAT3 up-regulation and increased GSH levels were associated with an earlier activation of the NGF/TrkA and mTOR signaling pathways in neurons ( Nerve growth factor is a neurotrophin (NT) that regulates neuronal development, differentiation, plasticity, cell death and survival (Lu et al., 2005)

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