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Other studies demonstrated a significantly decreased concentration of IGF BP-3 in patients with chronic hepatitis C as compared to healthy individuals (121)
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The Nde I restriction site upstream of torA was then removed by QuickChange mutagenesis (Agilent, Waldbronn, Germany) according to the manufacturers protocol using primers QC- Nde I-fw and QC- Nde I-rv (Supplementary table S3) resulting in the pPT plasmid
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