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The bacteria, yeasts, and fungi that make up our microbiome can be a source of beneficial byproducts such as short-chain fatty acids

J Exp Bot 60:12071218 Google Scholar Dueckershoff, K, Mueller, S, Mueller, MJ Reinders, J (2008) Impact of cyclopentenone-oxylipins on the proteome of Arabidopsis thaliana
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Briefly, RAW 264.7 cells were washed three times with phosphate buffer saline (PBS, Corning) and then incubated in ice-cold hypotonic lysing buffer (50 mM TrisHCl, 10 mM magnesium sulfate at pH 7.5) for 4 h at 4 C
