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Scalise et al
As markers of oxidative stress, 4-HNE and MDA are among the most potent reactive aldehydes (49)
However, persistent energy crisis blocks autophagy flux, preventing clearance of damaged mitochondria and protein aggregates [142]
Lactate dehydrogenase (LDH) release and ATP levels confirmed these protective effects, showing a reduction in LDH release from 212.29% to 122.98% and restoring mitochondrial membrane potential to 94.62% at 50 M of the puerarin-loaded PLGA nanoparticles
