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Since B12 is required for both the methionine synthase reaction and the methylmalonyl-CoA mutase reaction a megaloblastic anemia resulting from B12 deficiency is also associated with an accompanying methylmalonic acidemia, whereas megaloblastic anemias caused by folate deficiency are not

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At the time points 0, 2, 4, and 6 h, culture supernatants from different treatments were serially diluted, and then spotted onto LB agar