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Small 18 , e2105160 (2022)

Cagrilintide, the second generation (2020s) Novo Nordisk took the Pramlintide concept and applied the same half-life extension strategy that they used with Semaglutide: Kept the proline substitutions (anti-amyloid) Added a C20 fatty diacid via a -Glu linker to a lysine residue, albumin binding Additionally stabilized the N- and C-terminal positions Result: Cagrilintide with a half-life of 6.6 days (vs 50 minutes for Pramlintide)

Lessons from chronic intermittent and sustained hypoxia at high altitudes

If endotoxin is present, the activated enzyme cascade cleaves the substrate, producing a color change (typically yellow) whose intensity is proportional to the endotoxin concentration