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Titration protocols therefore hold a dose until nausea and other gastrointestinal effects settle rather than escalating on a fixed target date
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Genotyping PCR analysis Genomic DNA was extracted using the Quick gDNA Miniprep Kit (ZYMO Research, USA) followed by PCR amplification of the targeted region in the ABCC1 gene using LA Taq DNA Polymerase (Takara Bio, Japan) according to standard laboratory protocols
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