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The membrane was then incubated with appropriate horseradish peroxidase-conjugated secondary antibodies (1:5000)

Vitamin B12 absorption begins in the mouth but mainly occurs in the stomach and small intestine with the help of intrinsic factor, a protein that helps your body absorb B12. Weight loss surgery results in anatomical changes, such as a smaller stomach, reduced stomach acidity, and changes to the small intestine, that can lead to vitamin B12 malabsorption and decreased intrinsic factor secretion

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