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The expression plasmid pCGPx3M encoding the human GPx3 mutant was constructed by subcloning the Nde I/ Hin d III fragment from pUC57-GPx3M into the Nde I/ Hin d III sites of the pCold I vector (TaKaRa)

For the formation of xenograft tumours without ferrostatin-1 (Extended Data Fig
Arginine deiminase as a novel therapy for prostate cancer induces autophagy and caspase-independent apoptosis