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TM2, in particular, adopts a conformation in the orforglipron-bound structure that would be difficult to predict computationally, highlighting the receptor's conformational flexibility and the inherent limitations of structure-based drug design for class B GPCRs

The glycerol concentration in the medium was determined enzymatically using the recombinant glycerol kinase (Saci_2033, see below), pyruvate kinase (PK) and lactate dehydrogenase (LDH) (both from rabbit muscle

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