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The LET7 PCR products were digested by BamHI and StuI and ligated into the plant protoplast expression vector pHBT under a CaMV 35S ( p35S ) promoter with an HA, FLAG or GFP tag at the C terminus to obtain pHBT -p35S :: LET7-HA , p35S :: LET7-FLAG or pHBT -p35S :: LET7-GFP

V., Zambetti, G

Additionally, these novel inhibitors may provide increased selectivity and prolonged therapeutic effects, addressing a significant limitation of existing treatments

In lung cancer cells, the disruption of iron metabolism is characterized by upregulation of iron transport proteins such as transferrin (Tf) and hepcidin, while the expression of ferroportin, an iron export protein, is reduced ( Figure 1 )