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The muscle was hydrolyzed in 2 N HCl for 2 h at 95 C with constant agitation, followed by neutralization with 2 N NaOH
A major functional axis linking microbiota to metabolic regulation is the production of short-chain fatty acids (SCFAs)acetate, propionate, butyrate, and lactatevia anaerobic fermentation of dietary fiber
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Fe 2+ regulates multiple processes, such as oxygen transport and iron-sulfur (Fe-S) assembly in mitochondria [22, 25]
