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Advanced technologies for differentiating kidney organoids from hPSCs and efficient genome-editing systems with the clustered regularly interspaced short palindromic repeat CRISPR-Cas9 have enabled modeling of human kidney diseases 15,16,17

Briefly, 4 g peptides were, respectively, re-suspended with 16 l solvent A (0.1% formic acid in water), and separated by an EASY-nLC1000 system (Thermo Fisher Scientific), and then analyzed by a quadrupole-Orbitrap mass spectrometer (Q-Exactive Plus) (Thermo Fisher Scientific) equipped with an online nano-electrospray ion source

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