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Time to deproteinization influences some measures of GSH with additional differences between deproteinized heparinized and EDTA-treated plasma Noting the short half-life of free GSH within plasma and the possibility that heparin and EDTA differentially stabilize REDOX processes of GSH, we examined the impact of time to deproteinization and its interaction with type of anticoagulant used during sample collection on the measured levels of total GSH, free GSH, GSSG, or the ratio of free GSH to GSSG within plasma

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Materials and methods Participant selection Regularly-menstruating (i.e., 2135 days in length) women who were medication-free, not medically ill (as assessed by history, physical exam, neurological exam, MRI, gynecological exam, pap smear within the last year, laboratory tests, and ECG), and not pregnant were recruited for the study