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Lysates were combined with 6 Laemmli SDS sample buffer (Boston BioProducts, BP-111R) containing 5% 2-mercaptoethanol (VWR Life Science, M131), then loaded onto 420% Criterion TGX pre-cast gels (Bio-Rad, 5671093)

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Pharmacologic targeting of epigenetic regulators has been shown to restore normal gene expression and sensitize AML cells, including LSCs, to both conventional and novel therapies (Manivannan et al., 2025)

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However, the combination of GHB and alcohol can be extremely dangerous and even life-threatening

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