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Certain biochemical and physiological functions of plant GSTs are characteristic of a given plant-pathogen interaction type (e.g., glucosinolate metabolism, detoxification of mycotoxins), while other functions may be common for several or all interaction types, e.g., the control of plant cell death (oxidative stress) by GSTs and regulation of plant GSTs by various hormones and transcription factors

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The resulting pellets were further homogenized in the Tris salt buffer with the addition of 210% SDS, transferred to ultracentrifuge tubes and spun at 163, 200 g and 10 C for 30 min to extract the insoluble fraction