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Complementary DNA (cDNA) was made by reverse transcription of RNA (2 g) using Moloney murine leukemia virus reverse transcriptase (Promega, Madison, MI, USA) and labeled with Biotin-16-2-deoxy-uridine-5-triphosphate (Roche Diagnostics, Indianapolis, IN, USA)

Reported in-vitro work has examined its influence on fibroblast behavior, cell migration, and collagen-associated processes, frequently using the scratch-wound (wound-closure) migration assay as a quantitative readout of monolayer repair kinetics

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