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Next, cell samples were lysed in a lysis buffer (1% SDS, 10 mM EDTA, 50 mM Tris, and protease inhibitor mixture, pH 8.0)

Then, 20 L of MTT solutions was added to each well followed by an incubation period of 3 hours, then the media were removed and 200 l of DMSO was added to dissolve the crystal and the absorbance was recorded at 570 nm (BioTek, USA) and cell viability was calculated as a percentage compared to cells treated with DMEM alone

Additionally, consuming other foods like Brussels sprouts, cauliflower, and kale can also provide the three key building blocks (glutamate, cysteine, and glycine) to manufacture glutathione naturally

Huperzine A and donepezil protect rat pheochromocytoma cells against oxygenglucose deprivation