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Mitochondrial thioredoxin-2/peroxiredoxin-3 system functions in parallel with mitochondrial GSH system in protection against oxidative stress

Operates at the nuclear protein-interaction level: Unlike metabolic or hormonal peptides that act on membrane receptors, FOXO4-DRI functions by disrupting nuclear protein interactions

Samples were run in duplicate on two HPLC columns: (1) InfinityLab Poroshell 120 HILIC-Z (2.1 100 mm, 2.7 m, Agilent) with UHPLC Guard Poroshell 120 HILIC-Z (2.1 5 mm, 2.7 m, Agilent) and (2) reversed-phase ZORBAX Eclipse XDB-C18 (2.1 100 mm, 1.8 m, Agilent) with UHPLC Guard ZORBAX (2.1 5 mm, 1.8 m, Agilent)

It also contains glycosaminoglycans (GAGs) such as chondroitin sulfate, which are complex carbohydrates that help form the lubricant that surrounds the joints