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Initially, 22 lancets were employed to extract tissue portions (80100 mg), which were subsequently weighed and crushed in liquid nitrogen
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Parental ZR-75-1 cells were cultured in Phenol-red free medium containing 10% Dextran-coated charcoal-stripped FBS (DCC) with increasing concentration of Tamoxifen, starting from 0.5 M initially, and finally up to 5 M in a period of about 6 months
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