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Cells from the cardiac digestion were stained (CD45 FITC, Miltenyi Biotech) and analysed using flow cytometry (LSRFortessa, BD Biosciences)
Notably, neutrophils contribute to malignant progression of tumor 13,14,15,16,17,18
Since IGF-1R and IR are structurally similar and IGF-1 can bind to IR with lower affinity, IGF-1 could cause hypoglycemic effects if it can freely access to the IR [101,102]
434,435,436 Glycogen synthesized from glycolysis-derived G6P also repolarizes macrophages to an inflammatory phenotype via the pentose phosphate pathway and UDPG-P2Y14 signaling pathway
