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Moreover, the acidic assay conditions (pH 3.6) can precipitate proteins (e.g., milk casein) and generate pro-oxidant Fe 2+ ions, which may propagate radical chain reactions (Pulido et al., 2000
For total Ig detection from cell supernatants, plates were coated at 4 C overnight with goat Anti-Mouse Ig (Southern Biotech 1010-01) at 1 g/mL
Spray format is used in research applications where a pre-mixed solution is preferred
[DOI] [PubMed] [Google Scholar] 355.Thomson CD
