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Size analysis of liquid-like condensates using microscopy Droplet formation was triggered by adding 4 mM CaCl 2 to PDIA6 solutions containing 50 M PDIA6 in 50 mM HEPES-NaOH (pH 7.2) at room temperature with or without 10 mM NaCl, and PDIA6 droplets were observed within 30 min using a confocal microscope (FV1000, Olympus)

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Any deficiencies, dysbiosis, or other issues identified via functional lab testing should be addressed, along with associated conditions that may contribute to histamine intolerance