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Description
For total protein determination, tissue lysates were solubilized in Laemmli buffer (BioRad, Hercules, CA, USA) and separated by SDS-PAGE using precast TrisHCl gels (BioRad, Hercules, CA, USA)

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This is a complex syndrome that includes the following symptoms besides pain: fatigue, sleep disturbances, and functional symptomsmedical conditions with no structural or pathological cause [1]
