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Take extra care not to touch the vial's rubber diaphragm, which must stay sterile

Am J Physiol Renal Physiol 295:F388F396 Mulay SR, Kulkarni OP, Rupanagudi KV, Migliorini A, Darisipudi MN, Vilaysane A et al (2013) Calcium oxalate crystals induce renal inflammation by NLRP3-mediated IL-1 secretion

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Nonetheless, systematic population-based data are lacking to determine whether this axis exhibits nonlinear or bidirectional regulatory characteristics across different ages, metabolic backgrounds, and redox states