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LCMS/MS analysis of phosphorylated Ycf1 To measure quantitative phosphorylation of purified Ycf1, 10 g of Ycf1 were Trypsin/LysC (Promega, Madison WI) digested with an S-Trap column (ProtiFi, Farmingdale NY) using the manufacturers suggested protocol following reduction with DTT and alkylation with IAA with ProteaseMax (0.1% Promega) added to the digestions

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Apart from their primary function in phagocytosis and pathogen killing, neutrophils have also been linked to the development of granulomas in many infectious illnesses