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Assessment of mechanical allodynia in CCI rats After habituation of rats in plastic cages located on a metal wire mesh floor (1 h/day for two consecutive days), paw withdrawal threshold was measured as the average of three measurements with 3 to 5 min interval between applications using electronic von Frey apparatus (panlab, Harvard, Spain)

[25] For ovulation induction in women, a single IM dose of 5,000 to 10,000 IU is administered after follicular maturation, usually 24 to 48 hours before scheduled ovulation or insemination