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In 2001, BASF and Takeda (Japan) formed a joint venture and optimized the biotechnological production of vitamin B2 using the fed-batch method with vegetable oils as carbon sources and soy/corn products as nitrogen sources ( A

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The first step in the reaction is catalyzed by GCL, a heterodimer made up of a catalytic subunit (GCLC) that possesses the enzymes active site and performs the actual amino acid linkage, and a modulating subunit (GCLM) that regulates the activity of GCLC, as reviewed in Figure 1

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