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Description
33 , E335E341 (1984)

Application of ICH M7(R1) An assessment of the mutagenicity of impurities is needed because mutagenic chemicals such as alkylating agents are often used in the synthesis of MSP

13 C 6 -Glucose tracing metabolomics Cells were cultured in complete NB medium (1 10 6 cells/T-25 flask), without D-glucose supplemented with 4.5 g/L 13 C 6 -glucose (Sigma) and incubated at 37 C for 24 h

Freeze water in plastic containers with a couple inches (a few centimeters) of head space, as the expanding ice can break glass or overfilled containers
