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Even at the level of the median eminence, specific binding sites for IGF-I have been identified where it acts by inhibiting GHRH secretion [161, 162]

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In addition, there are various other extraction strategies, like Immunoaffinity capture, ultra-filtering or size-exclusion chromatography (SEC), co-precipitation methods, and microfluidic advancements, that have been effectively utilized in the case of mammalian EXOs (Le Gall et al., 2020)
