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The RT-qPCR analysis was performed on 100 ng of total RNA using iTaq Universal SYBR Green One-Step Kit (Bio-Rad, CA)

10.1371/journal.pone.0116407 4 Author Anonymous (2021)

Importantly, anti-SARS-CoV-2 antibody development after vaccination are not hindered by anti-TNF- use [107], as has been reported with rituximab, in patients with rheumatic diseases [108, 109]

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