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For long-term storage beyond one year, or for vials from a multi-month supply that will not be opened for extended periods, the freezer at -20 degrees Celsius is the better choice, extending stability to two years or more
It lacks electrolytes and is hypotonic, which can cause red blood cells to swell and rupture (hemolysis) if administered directly into the bloodstream
cDNA-containing ES clones were selected with neomycin, and presence of the cDNA was validated by PCR and with anti-Flag immunoblots from Cre-transfected clones
