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The sections were stained with hematoxylin and eosin and images were taken with a Nikon ECLIPSE Ni Upright Microscope (Nikon, Melville, NY) and ONL thickness at six locations relative to the optic nerve were measured: 25% (S3), 50% (S2), and 75% (S1) of the distance between the superior pole and the optic nerve and 25% (I3), 50% (I2), and 75% (I1) of the distance between the inferior pole and the optic nerve

The skin is cleaned with an antiseptic wipe at the injection site, usually the upper outer arm, and the intramuscular injection is given with a fine needle in around ten seconds

doi: 10.1093/bib/bbab120 57 HeRLeesonAAndonovALiYBastienNCaoJet al