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After several washes with distilled water, bands corresponding to PDHc-E2 (~75 kDa) or OGDHc-E2 (~55 kDa) were excised using a sterile scalpel and transferred to Protein LoBind tubes (Eppendorf)

But he talks about the kind of lack of motivation which many people have described anecdotally, like, okay, lowered their food drive, but lowered their drive period