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Subsequently, ROS reacts with PUFAs to deprive the hydrogen atom between its long chain double bonds, causing lipid peroxidation and ultimately leading to ferroptosis [31, 32]

More recently, a new LC-MS/MS method was developed, where compounds were separated by liquid chromatography using a Hypercarb column, particularly suitable for small polar compounds [43]

Animals were euthanized 1 h after caffeine injection, and brain tissues were biochemically examined for oxidants and antioxidants

In prdx-2 ( gk169 ) mutants, loss of peroxiredoxin activity leads to an increase in de novo GSH synthesis compared to wild-types, with an 2-fold increase in gcs- 1 mRNA and in GSH levels [173], possibly indicating a compensatory response by the de novo GSH synthesis network