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Description
Transient transfection of the PX459-sgRNA into the target cancer cell lines was performed using the Lipofectamine 3000 Transfection Reagent (Invitrogen) in 6-well plates (Corning Falcon) followed by puromycin selection (Gibco, 15 g/ml) for 3 days

Curr Opin Gast 18 : 711716 Furuse M et al
Ben reported feeling fantastic, with an immediate improvement in energy and vitality
Houghton, J., Morozov, A., Smirnova, I
