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You may also be more likely to develop vitamin B-12 deficiency if you have: Atrophic gastritis, in which your stomach lining has thinned Pernicious anaemia, which makes it hard for your body to absorb vitamin B12 Conditions that affect your small intestine, such as Crohns disease, celiac disease, bacterial growth, or a parasite Immune system disorders, such as Graves disease or lupus Been taking certain medications that interfere with the absorption of B12

The abundance of mechanistic in vitro and in vivo scientific data makes it challenging for non-expert clinicians to identify the optimal care for patients

Proteins were eluted by heating in SDS loading buffer at 90 C for 5 min, and lipoylation phenotypes were verified by immunoblotting the eluate alongside the input control