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In the matrix, the metallo--lactamase-family RNase ELAC2 requires zinc for tRNA 3-end processing, a key step for mitochondrial translation and the assembly of respiratory complexes 85

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Both ESI (+) and ESI () modes used water (LCMS grade) as mobile phase (A) and ACN as mobile phase (B), and formic acid was used as mobile phase modifier (0.1% for ESI (+) and 0.01% for ESI ())