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Description
Protein determination of mucosal samples was performed using a commercial reagent (BioRad)
[DOI] [PMC free article] [PubMed] [Google Scholar] 461.Luo, L
Detection was achieved with compatible Alexa Fluor fluorescein-conjugated secondary antibodies (Invitrogen, Carlsbad, CA, United States)
After several rounds of heterogeneous refinement, 80,325 particles were used for subsequent NU-refinement, resulting in a 3.70 resolution of the ATD 3D reconstruction
