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However, across many checkpoint-focused AD studies that primarily target oxidative stress, A clearance, or neuroinflammatory checkpoints, the field has only rarely measured a complete ferroptosis signaturethat is, simultaneous assessment of iron-dependent lipid peroxidation together with GPX4/SLC7A11 and ACSL4/LPCAT3 alterations, combined with ferroptosis-selective rescue experiments (e.g., ferrostatin-1, liproxstatin-1, or FSP1 activation)

doi: 10.1016/j.brainres.2004.01.036

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The mRNA expression of the target genes was analyzed using SYBR Green PCR Master Mix (#4309155, Applied Biosystems, Foster City, CA, USA), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as an endogenous gene