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Over the years, methods have been refined into the most commonly used protocol today, which uses an acid/ethanol precipitation (to disrupt the IGF-1/IGFBP complex) followed by the addition of insulin-like growth factor-2 (IGF-2) to prevent the reformation of the IGF/IGFBP complex

24 Dietary Antioxidants Vitamins and Minerals Fruits and vegetables contain antioxidant vitamins, including vitamin C, vitamin E, and pro-vitamin A carotenoids, activities of which have been well-studied

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