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Description
The lysate with LLO was loaded onto 30 ml Ni-NTA Superflow resin (QUIAGEN, Germany) packed in a XK 16/200 column (GE Healthcare, USA), washed with wash buffer (20 mM MES/NaOH pH 6.5, 150 mM NaCl), and eluted with an imidazole gradient in 20 mM MES/NaOH pH 6.5, 150 mM NaCl, with 500 mM imidazole as the final concentration
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[DOI] [PMC free article] [PubMed] [Google Scholar] 1034.Kaplan M., Tendler Y., Mahamid R., Shiner M., Aviram M., Hayek T
"I take 50 units from a 10 mg/mL vial, which gives me 5 mg" is complete
