glutathione resin protocol PureCube Agarose 1 and 3ml with total column volumes of 1 GST-His purification: A Two-step Affinity
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Solis et al., 2002
Each GLRX5 contributes one cysteine ligand to the 2Fe2S cluster, while a second thiolate ligand coming from a GLRX5bound glutathione stably binds GLRX5 ( glrx5 deletion in yeast cells has dysfunctional phenotypes in both mitochondrial ISC de novo biogenesis and cytosolic ISC assembly (Muhlenhoff et al., 2003
