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The cells were washed twice with PBS and maintained in 70% glycerol

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The recommended daily intake (RDI) of vitamin B12 varies depending on age and specific conditions: Adults: 2.4 micrograms (mcg) Pregnant women: 2.6 mcg Breastfeeding women: 2.8 mcg For those with a diagnosed vitamin B12 deficiency, higher doses may be prescribed: Initial treatment: 1,000 mcg intramuscularly once a week for four weeks Maintenance: 1,000 mcg intramuscularly once a month Is It Possible to Overdose on Vitamin B12

Dosage Control: Considerations: Some people may have difficulty absorbing Vitamin B12 from tablets, especially if they have digestive issues